We would like to stain cells (plasma membrane or intracellular) for the HIV-1 env antigen and count the positives by flow cytometry and we need a reagent that binds equally well to a variety of env sequences from different clades and to engineered variants of env, e.g., full-length gp160 or shortened, e.g., gp140 extracellular domain. I was thinking that fluorochrome-conjugated soluble human CD4 might be suitable for this application. The alternative would be a broadly cross-reactive monoclonal anti-env antibody. Does anyone know of a good commercial source of soluble human CD4 (purified or fluorochrome-conjugated) or anti-env monoclonal antibody that might work for us? Thanks. Gib Otten Chiron Corp. 4560 Horton St., M/S 4.3 Emeryville, CA 94608 Telephone: 510-923-2965 Fax: 510-923-2586 mailto:Gillis_Otten@chiron.com
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