Robert, I would not mix blood groups if possible. It is less likely that you will get hemolysis, but you will definitely get red cell aggregation (agglutination). The only antibodies that you should concern yourself with are the "naturally" occurring anti-A and anti-B red cell antibodies. Antibodies against most other blood groups do not occur as frequently (only as a result of transfusion or pregnancy), so they should not be of major concern. Regarding leukocytes it is possible that anti-HLA antibodies in multiparous women can cause leukocyte lysis, activation and aggregation. However, if you have to mix blood try to mix the same ABO types. Ana K. Stankovic, MD, PhD Centers for Disease Control and Prevention Public Health Practice Program Office Division of Laboratory Systems Laboratory Practice Assessment Branch 4770 Buford Highway MS G23 Atlanta, GA 30341 phone: (770) 488-8131 fax: (770) 488-8275 -----Original Message----- From: MOVERARE, ROBERT [GSO/0454] [mailto:robert.moverare@pharmacia.com] Sent: Monday, March 11, 2002 5:29 AM To: cyto-inbox Subject: Lysis of blood cells? Dear Flowers, I have a rather basic question. Is it possible to mix blood samples from different donors without the problem of lysis of the blood cells due to complement-mediated cytolysis, i.e. if the donors blood groups are miss-matched? For example, if blood from an "AB, Rh+ donor" is mixed with blood from an "O, Rh- donor" and then left on the bench for some hours at room temperature for later analysis by flow cytometry. Could it be harmful for the cells (erythrocytes or leucocytes)? Thanks in advance, Robert Robert Movérare, Ph.D. Scientist Pharmacia Diagnostics AB SE-751 82 Uppsala Sweden e-mail: robert.moverare@pharmacia.com
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