Greetings, guys & gals! This is a question only peripherally related to flow (the answer will be DIRECTLY related!): Does anybody have any experience transfecting Clontech's fluorescent proteins into U937 cells? We've tried electroporation and lipid-based reagents, with pathetic results. The same vectors happily transfected into HEK293 cells, so I'm convinced the vector is good. Thanks for any suggestions. Andrew A. Beernink Research Scientist Manager, Flow Cytometry Novasite Pharmaceuticals, Inc. 11095 Flintkote San Diego, CA 92121 858) 638-8584
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