There are two ways. One is just to put the nodes through a mesh as you would with spleen. The other is with collagenase, DNAse and hyaluronidase. For that protocol, look at the procedures used by Yong-Jun Liu for human tonsil. Ron on 9/10/02 3:57 AM, Boros András dr. at a.boros@richter.hu wrote: > > Hi all, > > Would you be so kind to indicate a good source of lymphocyte cell separation > technique > for flow cytometry analysis. > I would like to carry out CD44 staining on lymph node cells of mice. > > Thank you wery much > > Andras Boros > Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.gov
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:25 EST