Hi Has anyone successfully managed to stain cells with bromodeoxy uridine and iododeoxy uridine antibodies and been able to distinguish between the two? Seems like it should be possible although there is some cross-reactivity between antibodies. If so which antibodies did you use and what blocking statagy if any did you use. We want to challenge an animal with antigen in the preence of BRDU, let it rest up a while then re-challenge with antigen in the presence of IRDU and see if the same cells respond. Can anyone think of another way to do this without using radio isotopes? Thanks for any suggestions. Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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