Hi flowers, I am trying to work out a
protocol for acquisition of cells labelled with CFSE and 3 more colors (PE, ECD and
PE-Cy5). These are PBLs stimulated with mitogens and antigens. The problem id that CFSE
gives a staining so bright (in the fourth log decade) that everything else (specially PE)
is completely contaminated by the strong CFSE signal. I tried last friday to compensate
for PE channel without succes (I run out of cells to acquire). I am going to try again
today. Does anyone have any experience working with CFSE and other colors? Is tehre
anyway to find out a cytosettings for this combination?
Many thanks
Jose Miguel Benito
Madrid
Spain