Jose, It is possbile to use 4-color comb on XL. I used to do IFNg-PE, CD3-ECD, and 69-PC5. Other combs are also possible. Obviously, this all depends on how strong cells are labeled with CFSE. If you have money to invest, you should get a PC7 filter kit, and this will make you life so much easier. If you still want to voltage setting, 4-color comp, and CFSE-labeling for 4 color, please contact me. Aki Hoji University of Pittsburgh Dept. Infectious Diseases and Microbiology Rm 526 Parran Hall, GSPH-IDM, 130 Desoto Street, Pittsburgh, PA 15261 Tel: 412-624-3072 Fax: 412-624-4953 > > From: Jose Benito <jbenito1@hotmail.com> > Date: Mon, 26 Aug 2002 14:49:51 +0200 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: CFSE analysis by flow > > Hi flowers, I am trying to work out a protocol for acquisition of cells > labelled with CFSE and 3 more colors (PE, ECD and PE-Cy5). These are PBLs > stimulated with mitogens and antigens. The problem id that CFSE gives a > staining so bright (in the fourth log decade) that everything else (specially > PE) is completely contaminated by the strong CFSE signal. I tried last friday > to compensate for PE channel without succes (I run out of cells to acquire). I > am going to try again today. Does anyone have any experience working with CFSE > and other colors? Is tehre anyway to find out a cytosettings for this > combination? > Many thanks > Jose Miguel Benito > Madrid > Spain > > > Join the world’s largest e-mail service with MSN Hotmail. Click Here > <http://g.msn.com/1HM1ENXX/c157??PI=31901> >
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