Hi Richard, I would suggest that more rabbit antibodies would be used if they could be directly conjugated, either commercially or with the Zenon type of labeling. We routinely use several for intracellular staining of signal transduction molecules. Randy T. Fischer NIH/NIAMS Autoimmunity Branch Building 10, Rm 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: Richard Haugland > Sent: Monday, August 19, 2002 5:10 PM > To: Cytometry Mailing List > Subject: Rabbit antibodies in flow cytometry > > <<File: dick.vcf>> > How common is it to use rabbit primary antibodies in flow cytometry > (versus mouse antibodies). I suspect very uncommon. We are trying to > determine whether it would be useful to test our Zenon antibody labeling > method in flow or only imaging and blots. > > thanks > > > >
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