We had stubborn compensation problems with the PE-Cy5 and APC. It was due
to the red laser's misalignment. FacsComp did not reveal it. It is using
PerCP and that still could be compensated. The best remedy was to run a
single stain CyChrome (PE-Cy5) control while the field engineer aligning
the laser.
Best regards, Akos (Biogen)
Joseph Webster <J.Webster@centenary.usyd.edu.AU>
07-Nov-2002 04:41 PM
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
cc:
Subject: Re: Pe-Cy5 and APC
Yes, they are used OK here.
You don't detail the problem, so this may or may not be relevant:
Tandem dyes such as PE-Cy5 are more fragile than the simpler ones,
will give highly variable results if the PE and Cy5 components get
partially separated.
This can be caused by exposure to light &/or heat, and shows up as
variations in compensation and intensity.
Cheers, Joseph.
At 02:35 7/11/2002, Julie Nelson wrote:
>Hi ya'll,
>Has anyone successfully compensated PE-Cy5 (FL3) and APC (FL4) on a
>FACSCalibur? I have tried both during acquistion and post-aquisition
>and have not had much luck. I am currently not recommending it, but I
>am curious as to why it doesn't seem to work.
>Thanks,
>Julie
--
Joseph Webster, Flow Cytometry Facility
Centenary Institute, Sydney, AUSTRALIA.
Phone +61-2-9565-6110
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:29 EST