Hi all, A question about activation of lymphs. When I activated lymphocytes using PMA and ionomycin, I see a population of cells with reduced side scatter properties apart from the regular lymphocytes. It looks like there are two populations (parallel shift) based on SSC. The shift is towards the left, and the number of cells shifting increases with activation-time. Has anyone observed this? What is it due to? Do the cells (lymphs) shrink upon activation? thanks, Ragoo *********************************************************** Arumugham Raghunathan, Ph.D Flow Cytometry Group Cellular Analysis Section Molecular Staging Inc., 300 George Street, Suite 701 New Haven, CT 06510 Tel# 203-772-5074 Fax# 203-776-5278 *************************************************************
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