Dear Flow Cytometry list reader, as a disclaimer, I'm quite new to the FCS techniques, and hoping not to upset anyone with my question. I looked through the archive but couldn't find a answer. I just discovered the the software package "Summit" form Cytomation, which is "free" to use as a offline analysing tool. I was surprised to read the following statement in the online tutorial: " By using the Summit software, there is no need to adjust compensation on the cytometer before acquiring data. Summit provides the ability to adjust compensation after the raw data has been acquired and saved as a file" What is your opinion to this statement ?. Is this true ?. FlowJo, FCSPress also give the possibility to "software" compensate samples. Will I get the same result when I do the compensation with these packages, or will the result depend on the underlying algorithmen ?. I appreciate if anyone can comment on my questions. I'm also interested to know if someone knows review articles on compensation/how to do (software and hardware). Many many thanks Frank Mattes PhD student Royal Free Hospital and University College Medical School London f.mattes@ucl.ac.uk __________________________________________________ Do You Yahoo!? Yahoo! - Official partner of 2002 FIFA World Cup http://fifaworldcup.yahoo.com
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