Hello Frank, Yes! The statement what is written in the Summit software tutorial is true. Moreover I think it's a advisable in the case of newbies in color compensation because the software does not make mistake. I can recommend strongly the next website made by Mario Roederer : http://www.DRMR.com/compensation/index.html Here, you will find everything you need to know about the color compensation. But it's also recommended to read the tutorials of the softwares you mentioned. In the case of FlowJo I compared my result using the "old method" compensating during acquiring and the "new method" compensating after acquiring and I got the same results. The latter has the plus ability that makes possible to compensate between such channels like FL1 and FL3 and not only between neighbouring channels like FL1-FL2 or FL2-FL3. The procedure before making a new compensation is almost the same: you need to acquire data for unstained and one-color-stained samples. Using the "after-acquiring" method you can also store your compensation matrix and apply to other samples which are similar in their fluorescent characteristics. Good luck, Pal Jakso University of Pecs Faculty of Medicine Dept. of Pathology 7643, Hungary 12. Szigeti str. On Wednesday, June 12, 2002, at 01:01 , Frank Mattes wrote: > > Dear Flow Cytometry list reader, > > as a disclaimer, I'm quite new to the FCS techniques, > and hoping not to upset anyone with my question. I > looked through the archive but couldn't find a answer. > > I just discovered the the software package "Summit" > form Cytomation, which is "free" to use as a offline > analysing tool. I was surprised to read the following > statement in the online tutorial: > > " By using the Summit software, there is no need to > adjust compensation on the > cytometer before acquiring data. Summit provides the > ability to adjust compensation after the > raw data has been acquired and saved as a file" > > What is your opinion to this statement ?. Is this true > ?. > FlowJo, FCSPress also give the possibility to > "software" compensate samples. Will I get the same > result when I do the compensation with these packages, > or will the result depend on the underlying > algorithmen ?. > > I appreciate if anyone can comment on my questions. > I'm also interested to know if someone knows review > articles on compensation/how to do (software and > hardware). > > Many many thanks > > Frank Mattes > PhD student > Royal Free Hospital and University College Medical > School > London > f.mattes@ucl.ac.uk > > __________________________________________________ > Do You Yahoo!? > Yahoo! - Official partner of 2002 FIFA World Cup > http://fifaworldcup.yahoo.com > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:12 EST