Sorry for having upset Calman or caught him at a bad moment. If you spent some time to read the manual on their website you will find that they first of all publishes the ingredients for the test including the antibodies (but not their clone / source, which makes a difference) and the relevant background literature. Apart from fmlp the kit includes an appropriate buffer, grass mix and a selected mite allergen. Thus there is no need to feel hoodwinked as this is not just a reagent with a misleading name but a nearly complete assay. Otherwise they would probably sell you the apyrogenic water as a concentrate with a funny name. As I have to judge my time against the expense I have to consider if I want to reinvent an assay or if it is cheaper to buy one ready to use. For that you need to judge the time it takes you to select and titrate the appropriate antibody and set up the controls, check expiry dates... Depending on the quantity of tests I need to do and the frequency it might be worthwhile to set up my own test, including all the QC. If I run a clinical routine lab with lots of allergy testing that might well be the case. I would even have to think about starting to conjugate my own reagents - or change supplier, but for me as the "occasional user" I would waste far to much money by doing so. Gerhard Nebe-von-Caron Research Scientist SEAC - Safety and Environmental Assurance Centre Applied Science & Technology Group Unilever Colworth, Sharnbrook, Bedfordshire, MK44 1LQ, UK Tel: +44 (0)1234 264822, Fax: +44 (0)1234 222552 E- mailto:Gerhard.Nebe-von-Caron@unilever.com P.S. don't tell Unilever, but if you want to make up your own washing powder you can try http://www.hobbythek.de/archiv/305/ - if you can speak German or http://www.naturalhomemagazine.com/recipe/misc%20recipes/laundry.htm for some less scientific approach. Hope this helps you to save the odd $0.02 -----Original Message----- From: Calman Prussin [SMTP:CPRUSSIN@niaid.nih.gov] Sent: Monday, April 08, 2002 5:41 AM To: Cytometry Mailing List; 'Gerhard Nebe-von-Caron'; 'vannacci@pharm.unifi.it' Subject: RE: was Human IgE, now "why Basotest?" You can also just buy some CD63 mAb and some f-Met-Leu-Phe (positive control). There is no magic to the "Basotest", as it is based on surface detection of translocated/exocytosed CD63 in anti-IgE gated basophils. Depending on how much anti-IgE you use for your activation, you may find that you are no longer able to detect basophils with flurochrome labeled anti-IgE. If that is the case, CD123 (IL-3R alpha) works just about as well to identify basophils (gate on CD123high cells). I guess I have pet peeve about trade names. Somehow I can't take it seriously when people mention they used "Golgi-plug" rather than just say "Brefeldin A"....or is that "Golgi-stop"??? Just my $ 0.02, Calman ---------- From: Gerhard Nebe-von-Caron Sent: Friday, April 5, 2002 11:13 AM To: cyto-inbox Subject: RE: Human IgE <<File: ATT01064.ATT>> You might want to try the Basotest (http://www.orpegen.de/diagnos0.htm) It probably does what you want and includes the relevant control material. Good luck Gerhard Nebe-von-Caron Research Scientist SEAC - Safety and Environmental Assurance Centre Applied Science & Technology Group Unilever Colworth, Sharnbrook, Bedfordshire, MK44 1LQ, UK Tel: +44 (0)1234 264822, Fax: +44 (0)1234 222552 E- mailto:Gerhard.Nebe-von-Caron@unilever.com -----Original Message----- From: Alfredo Vannacci [SMTP:vannacci@pharm.unifi.it] Sent: Wednesday, April 03, 2002 1:21 PM To: cyto-inbox Subject: Human IgE Dear flowers, I was wondering if anyone of you knows a company from which I could buy policlonal human IgE. I need them because we are studying human basophil activation by flow cytometry and we wish to standardise their response to anti-human IgE by saturating cell surface FcE-RI receptors. Thank you in advance for your help. Dr Alfredo Vannacci, MD University of Florence Department of Preclinical and Clinical Pharmacology email: vannacci@pharm.unifi.it tel: +39(0)554271234 fax: +39(0)554271280
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:06 EST