Manickam Janakiraman wrote: > > Dear Flow users, > > I am new to the flowcytometry technique. I > intend using it for the detection of apoptosis in mouse embryo > fibroblasts. I tried some control and staurosporine treated cells in the > FACSCalibur after staining with PI. Contrary to my expectation the DNA > histograms looked different. There was a peak at hypodiploid position in > the all the samples measured , even in control ones. The hypodiplod sub-G1 peak is a natural sign of apoptosis if you stain with PI after permeabilization. You can control the unusual observation namely that your control sample also shows a hypodiploid sub G1 peak is applying of other apoptosis detecting methods along the PI. Otherwise are you sure that your control cells does not exhibit apoptosis ? > I analysed the dot > plots and i could see the difference in apoptotic and live cell > population clearly. What kind of dot plots do you use? Could you present some dot plots and PI histograms copying or attaching them into an email? Best regards, Pal Jakso University of Pecs Faculty of Medicine Dept. of Pathology 7643, Hungary 12. Szigeti str.
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