I have found that lignocain will make adherent macrophages fall off plastic - I haven't used the technique for quite a while, but I recall there are publications in the area, so a medline search might turn up details of the technique. Robin Barclay ----- Original Message ----- From: "Dee Harrison-Findik" <duygu.harrison-findik@imvs.sa.gov.au> To: cyto-inbox Sent: 22 February 2002 02:09 Subject: harvesting cultured macrophages > > Dear Flowers > I am isolating macrophages from human blood by the simple adherence > technique. > I am having difficulties to harvest the adhered macrophages by conventional > trypsinisation. Majority are still stuck to the TC. flask. Cell scraping > does not yield very happy cells, either. > Is there any specific reagent or technique which will lift off all (or > majority) of the adhered macrophages as viable as possible ? > Any input is greatly appreciated. > Cheers > Dee > > >
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