Dear Flowers I am isolating macrophages from human blood by the simple adherence technique. I am having difficulties to harvest the adhered macrophages by conventional trypsinisation. Majority are still stuck to the TC. flask. Cell scraping does not yield very happy cells, either. Is there any specific reagent or technique which will lift off all (or majority) of the adhered macrophages as viable as possible ? Any input is greatly appreciated. Cheers Dee
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