We ordered a Calibur over a year ago WITH the FACSFlow included in the
quote. BD could not deliver the FACSFlow for a year. We were told by our
rep that it was not available in the US. That's the last time we heard
about it.
Akos
"Richard Konz" <RKonz@wyeth.com>
16-Jul-2002 07:54 AM
To: Akos_Szilvasi@biogen.com, cytometry@flowcyt.cyto.purdue.edu
cc:
Subject: Re: Instability with large sheath tank
Akos and Gary:
Actually, I have three of the FACSflow systems on order from BD
Biosciences. They are supposed to be available
for sale in the US in a month or so. They are available for the 'Calibur
or can be retrofitted for FACScans.
Contact your BD rep. for details.
-Rich
Richard F. Konz, Jr.
Scientist
Manager, Flow and Image Cytometry Facility
Wyeth
200 Cambridge Park Drive
Cambridge, MA 02140 USA
Office: 617-665-5522
Lab: 617-665-5551
>>> "Akos Szilvasi" <Akos_Szilvasi@biogen.com> 07/09/02 02:02PM >>>
Gary, sometimes the plastic tanks develop very small leaks that hard to
detect. Try a new one and see if it makes a difference. We have a BD
FACSFlow system that can use 20 liters tanks. Great addition to the
Calibur BUT you can not buy it. They do not sell it in the US. Try Cytek
(http://www.cytekdev.com). They have a large tank supply system for the
FACSCan/Calibur.
Best regards, Akos
Biogen
Gary.A.Ward@dartmouth.edu (Gary A. Ward)
05-Jul-2002 10:35 AM
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
cc:
Subject: Instability with large sheath tank
Hello Flow Network,
I'm looking for advice from anyone with experience using a FACScan or
FACSCalibur with a large sheath tank. We have been experiencing
instability
with our fluorescent signals vs time as well as an apparent decrease in
fluorescence intensity. I've checked for air leaks at the tank and
believe it
to be stable. I've also checked for air bubbles in the Pall filter and
tubing
and these seem to be clear. We routinely clean our system so I don't
believe it
to be dirty. The history of the laser indicates it to be stable. Any
other
suggestions?
Also what PSI air pressure are users running with the large tanks?
Thank you!
Gary A. Ward
H C Englert Cell Analysis Laboratory
Norris Cotton Cancer Center
Lebanon, NH USA
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:15 EST