Good morning Flow-ers ! Just a quick query on single cell suspension prep for Flow: Am about to try making cell suspensions from tumor and aortic tissue for Flow with fluorescent membrane antibodies. My question is whether it's better to mechanically disaggregate tissue or enzymatically (collagenase etc). Does enzymatic tretatment have any negative effect on cell surface antigens ? Many thanks as always, Annette Annette Byrne PhD Scientist Pharmacyclics 995 E Arques Ave Sunnyvale CA 94085-4521 USA Tel: 001-408-3283640 Fax: 001-408-3283689 e mail : AByrne@pcyc.com www.pharmacyclics.com
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