More common that certainly I would like. Even affinity purified rabbit ab are a pain, but sometimes that is all that is available for some ligands and receptors. The background is quite high, and I would wonder whether the Zenon system might help with that. Please test it for flow. ron on 8/19/02 5:10 PM, Richard Haugland at richard.haugland@probes.com wrote: > How common is it to use rabbit primary antibodies in flow cytometry > (versus mouse antibodies). I suspect very uncommon. We are trying to > determine whether it would be useful to test our Zenon antibody labeling > method in flow or only imaging and blots. > > thanks > > > Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.gov
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