Dr. Haugland,
I for one would love to be able to use your Zenon reagents with rabbit
antibodies. Although I agree that I don't use them as often, sometimes
the only the Ab available is raised in rabbit. And then my choices of
fluor are limited by the available secondaries. Normally, to get the
color I want I have to do a three step stain! Rabbit primary (unlabeled),
biotinylated secondary, SA-fluor tertiary. I've used the Zenon reagents
frequently since they came out and it would be great to have them
available for other non-mouse Abs. This is becoming more of an issue now
that we are starting to routinely do 5+ color experiments. (We actually
have several 8-color experiments on the drawing board.) The Zenon
reagents are a great alternative to custom conjugations!
David McFarland
GlaxoSmithKline
----- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 21-Aug-2002 10:15
-----
"Richard Haugland" <richard.haugland@probes.com>
19-Aug-2002 17:10
To: "Cytometry Mailing List"
cc:
Subject: Rabbit antibodies in flow cytometry
How common is it to use rabbit primary antibodies in flow cytometry
(versus mouse antibodies). I suspect very uncommon. We are trying to
determine whether it would be useful to test our Zenon antibody labeling
method in flow or only imaging and blots.
thanks
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