Dr. Haugland, I already have plans for the mouse Zenon. I would also be interested in Zenon for rabbit antibodies. How about Zenon for human antibodies? Todd Todd Belanger Vaccinex, Inc. -----Original Message----- From: David.C.McFarland@gsk.com [mailto:David.C.McFarland@gsk.com] Sent: Wednesday, August 21, 2002 10:25 AM To: cyto-inbox Subject: Rabbit antibodies in flow cytometry Dr. Haugland, I for one would love to be able to use your Zenon reagents with rabbit antibodies. Although I agree that I don't use them as often, sometimes the only the Ab available is raised in rabbit. And then my choices of fluor are limited by the available secondaries. Normally, to get the color I want I have to do a three step stain! Rabbit primary (unlabeled), biotinylated secondary, SA-fluor tertiary. I've used the Zenon reagents frequently since they came out and it would be great to have them available for other non-mouse Abs. This is becoming more of an issue now that we are starting to routinely do 5+ color experiments. (We actually have several 8-color experiments on the drawing board.) The Zenon reagents are a great alternative to custom conjugations! David McFarland GlaxoSmithKline ----- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 21-Aug-2002 10:15 ----- "Richard Haugland" <richard.haugland@probes.com> 19-Aug-2002 17:10 To: "Cytometry Mailing List" cc: Subject: Rabbit antibodies in flow cytometry How common is it to use rabbit primary antibodies in flow cytometry (versus mouse antibodies). I suspect very uncommon. We are trying to determine whether it would be useful to test our Zenon antibody labeling method in flow or only imaging and blots. thanks
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