Hi Niga, Dot plots may be a little too "realistic" for you. Try using contour plots, since no one ever publishes what the contour lines mean, you can make very few cells look like nice beautiful populations :-) I will leave the moral implications of this strategy up to you to deal with..... -Dave -------------------------------------------------------------------------- God promised us a land of milk and honey, a land of vines and fig trees and pomegranates. That's what he promised us, and that's what he gave us, along with a restrictive set of dietary laws that have not made life easier. To the goyim He gives bacon, sweet pork, juicy sirloin, and rare prime ribs of beef. To us he gives pastrami. In Egypt we get the fat of the land, in Leviticus he prohibits us from eating it.... the fat is bad for our gallbladder. -King David, as quoted by Joseph Heller -------Original Message------- From: Nawroly, Niga Date: Friday, December 20, 2002 11:37:05 AM To: cyto-inbox Subject: help with dotplots Dear all One of our lab's searchers have been looking at gamma delta T cells (very low percentage of PBMCs). Now, he wants to present his data in dotplots (paper quality). The problem is that, he did not acquire enough cells to be able to see a nice population when it is printed out. My question is to know if there is a way by using a software to make the dots look more realistic (each dot representing one cell or half a cell!). PS: We use winlist, cellquest and WinMDI Have a merry Xmas and a happy new year :-) Many thanks in advance Niga Nawroly Head of flow cytometry Department of Respiratory Medicine Imperial College (St.Mary's) Norfolk Place Paddington London W2 1PG UK Tel: 020 7594 37 63 FAX: 020 7262 8913 .
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