Hi these cells have high autofluorescence. what are you trying to detect ? especially if its GFP, try lowering the PMTs so that your mock transfected cells all fall in the first decade, and be sure to exclude dead cells with PI. You can also perform autofluorescence compensation, to enhance your signal to noise. good luck! happy holidays to all, Rachel ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Anke Kretz > Reply To: kretz-rommel@alxnsd.com > Sent: Thursday, December 19, 2002 6:44 PM > To: Cytometry Mailing List > Subject: COS cells > > > We are trying to do FACS sorting on transfected COS cells. However, > even untransfected cells give us pretty high background. Cells are > detached with accutase. does anybody have experience with FACS of > transfected COS cells? > > thank you, > Anke > > >
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