Hi fellow flow-ers, Happy Holidays ! I would like to seek some advices from you for detaching fixed cells in 96 well TC plate(by formaldehyde and then permeabilized by MetOH) to run on BD multi-well autosampler. Is it possible to lift the cells off the well after fixation ? What would you recommend, trypsin, accutase or cell dissociation buffer to preserve surface receptor for Ab staining ? Thanks very much in advance for your help ! Albert Tai
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