Hi everybody, I know a lot of people are still on holidays but nervertheless I am posting my question again. I've got no replies yet. Dear experts, is there anybody who has experience or a good working protocol in preparing single cells from stomach carcinoma tissue or kidney carcinoma tissue for further flow cytometric anaylsis? I have a client who started to do this but the first attempts looked - let's say not as what we assume and expect is a nice single cell suspension for flow analysis. A lot of debris and a lot of aggregates also seen during counting the cells on the microscope. Even after filtering the 'suspension' I can't identify a certain cell population. And there also is no difference between permeabilized (for cytoplasmic antigen staining) and nonpermeabilized 'cells'. I appreciate any help Many thanks in advance Regards Volker (a little bit stressed about this isssue) Volker Eckstein PhD Dept. of Internal Medicine V Medical school of the university University of Heidelberg GERMANY volker_eckstein@med.uni-heidelberg.de
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