RE: NK cytotoxicity assay with human whole blood

From: Giovanna Borsellino (gborsel@tin.it)
Date: Tue Jul 02 2002 - 10:31:26 EST

Next message: maciej simm: "cytokine reference sheet"
Previous message: Richard Konz: "FRET frets..."
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Hello Lee,

we have recently purchased the fluorogenic cytotoxicity assay from
OncoImmunin (CytoxiLux) (see Nature Med. 8:185-189, 2002). I like the idea
that  you can combine the cytotoxicity assay with multiparametric flow
cytometry and characterize the effector cells in mixed populations (such as
PBLs). We haven't tried it yet, but it looks very neat, and I wouldn't be
surprised if it turns out to be more sensitive than 51Cr. I'll let you know.

Cheers,

Giovanna



------------------------------------------------
Giovanna Borsellino, M.D., Ph.D.
Neuroimmunology
IRCCS Santa Lucia
Via Ardeatina 354
00179 Roma
Italy

Tel. ++39.06.51501521 (office)
        ++39.06.51501552 (lab)
Fax  ++39.06.51501553

e-mail: g.borsellino@hsantalucia.it
            gborsel@tin.it

Next message: maciej simm: "cytokine reference sheet"
Previous message: Richard Konz: "FRET frets..."
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:14 EST