Hi, I've never gotten good CVs when staining yeast DNA with PI. A former colleague of mine had much better luck using mithramycin A, but you need a 457 line on your laser to get good excitation. For a brief protocol see: Frykman S, Srienc F. Cell cycle-dependent protein secretion by Saccharomyces cerevisiae. Biotechnol Bioeng. 2001 Nov;76(3):259-68. regards, Nick Abu-Absi Process Development Scientist Chiron Corporation 4560 Horton St. M/S M-200 Emeryville, CA 94608 Lab: (510)923-7421 Fax: (510)923-4116 -----Original Message----- From: Haug, Jeff [mailto:JSH@Stowers-Institute.org] Sent: Friday, August 23, 2002 4:42 PM To: cyto-inbox Subject: Yeast DNA staining Hi Everyone, I am looking for a good procedure for staining yeast DNA. I have tried an ethanol fix/PI procedure but the CV were in the teens. I plan to try using SYBR Green next week. Best regards, jsh Jeffrey S. Haug Managing Director, Cytometry Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 jsh@stowers-institute.com <mailto:jsh@stowers-institute.com> Lab- 816 926 4142 FAX- 816 926 2022
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