Annie, If you are looking at more than one color, I would advice you to use individual fluorescent tagged controls before looking at your test samples. Peter Mucheru. -----Original Message----- From: Annie Bang [mailto:banga@smh.toronto.on.ca] Sent: Friday, August 23, 2002 9:45 PM To: cyto-inbox Subject: Standardizing cytometer using fluorescent microspheres (target channeling?) We are using a FASCalibur to measure differences in binding of fluorescent-tagged antibody to "normal control" and "test" platelets. We would like to eliminate the need to use a large number of normal controls per assay to establish a 1% positive region for the histogram statistic. Could we set up the cytometer daily, using microspheres to set PMT voltages to give a consistent channel measurement. Then run 10 (or so) normal controls to establish the positive region and analyse all other test samples based on this historical positive region, no matter what day the test samples are run. Would this eliminate the need to process a parallel normal control for each test sample on each day? Is this even a valid procedure to follow? Any comments, suggestions, etc. are appreciated.
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