Dear Patricia, with the help our chemical safety officer this is what we decided to do: PFA waste in the waste tank (that is very diluted indeed) goes in the sink (after bleaching). Unused sample left in the tube (1% PFA) goes in a specific waste bottle that is kept till is full and is disposed as chemical waste. We do not autoclave any sample containing PFA since the high temperature could lead to production of toxic fumes that could be harmful for our service room personnel. I hope this help Mara Mara Rocchi Moredun Research Institute Pentland Science Park Bush Loan, Penicuik EH25 0PZ Scotland, UK Tel: +44-131-4455111 Fax: +44-131-4456111 e-mail: mara.rocchi@mri.sari.ac.uk -----Original Message----- From: Patricia Echeagaray [mailto:pechado@hotmail.com] Sent: 02 September 2002 16:40 To: cyto-inbox Subject: Paraformaldehyde disposal question All, At my current company, one of the scientists here insists that the waste from counting fixed cells on the coulter counter should be saved and disposed of as chemical waste. The cells (T'cells/PBMCs) are fixed in a final concentration of 1% PFA. 100 ul are added to a 10 ml volume of ISOTON. Can any of you tell me why...assuming this is common practice? At other places I've been in the past, I'm fairly sure we just bleached it and tossed it down the sink. That is what is done with the other 100 ul of fixed cells (where PFA is much more concentrated, of course) since we make 200 ul total volume (100 ul 2%PFA + 100 ul cells). Thank in advance! Tricia Echeagaray _____ Join the world's largest e-mail service with MSN Hotmail. Click Here <http://g.msn.com/1HM1ENUS/c157??PI=44364> The information contained in this e-mail message and any files transmitted with it is confidential and may be legally privileged. It is intended only for the addressee and others authorised to receive it. If you are not the intended recipient or the person responsible for delivering the message to the intended recipient you are advised that you have received the e-mail in error and that any disclosure, copying, distribution or action taken in reliance on the contents of the e-mail and its attachments is strictly prohibited and may be unlawful. Please advise the sender immediately by replying to this e-mail and delete it and all copies from your system.
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