All,
At my current company, one of the scientists here insists that the waste from
counting fixed cells on the coulter counter should be saved and disposed of
as chemical waste. The cells (T'cells/PBMCs) are fixed in a final concentration
of 1% PFA. 100 ul are added to a 10 ml volume of ISOTON. Can any of you tell me
why...assuming this is common practice? At other places I've been in the past, I'm
fairly sure we just bleached it and tossed it down the sink. That is what is
done with the other 100 ul of fixed cells (where PFA is much
more concentrated, of course) since we make 200 ul total volume (100 ul 2%PFA + 100
ul cells).
Thank in advance!
Tricia Echeagaray