Please, I need your help. I am going to be running flow on cells that are synchronized using nocazadole (diluted up in DMSO). I will have two time points, 15 hrs with the drug and 24 hours with the drug. These cells (adherent) will be stained with FITC conjugated anti-PARP (primary antibody) and then stained with PI. I will be doing these 2 drug time points on 5 different cell lines. The controls that I plan to use are: -cells with no DMSO -cells with FITC Ab only -cells with PI only -cells with FITC & PI (DMSO only) (no nocazadole) What other controls do I need ??? Do I need controls for both time points ??? PLEASE ADVICE. Thanks, P. Nunes Charlotte, NC perla.nunes@carolinashealthcare.org *********************************************************************** This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you.
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