Dear Maciej, I have contacted Prof. H. Weich at the German Research Centre for Biotechnology (GBF) since I knew he developed many antibodies and assays to study angiogenesis and herewith his answer: Concerning your question about VEGFR-1 antibodies I only know one monoclonal which can be used for cell sorting: 101-M26 from RELIATech. You can download the data-sheet from www.reliatech.de <http://www.reliatech.de> and if necessary I can fax you the results by sorting HUVEC and PAE/flt-1 cells with this antibody. Herbert A. Weich, Ph.D. E-mail: weich@gbf.de <mailto:weich@gbf.de> http://www.gbf.de <http://www.gbf.de/Welcome.html> Hope this helps, best regards, John John Voorn Sanquin Reagents Product manager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: maciej simm [SMTP:flowlab@myflow.net] Sent: Tuesday, May 14, 2002 4:23 PM To: Cytometry Mailing List Subject: VEGF-R1 (FLT-1) antibodies for flow? Dear colleagues, I'm looking for a reagent that will recognize the human VEGF-R1 (FLT-1) by flow. Does anyone have a good source? References would be useful as well. Thanks! Maciej -- Maciej S. Simm Senior Technologist Weill Cornell Medical College Pediatrics Hematology/Oncology Immunology Laboratory/Flow Cytometry Unit 525 East 68th Street N-805 New York, NY 10021 voice (212)746 3428 fax (212)746 8573 --
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