Greetings, Can someone tell us if steric hindrance will play a role in a 3-color experiment in which human erythrocytes/retics are stained simultaneously for CD49d (VLA, very late activation), CD36 (erythrocyte adhesion receptor), and AnnexinV, if one of the antibodies/reagents is labeled with (the very large molecule) phycoerythrin and another with one of the PE-Cy5 reagents.? I've seen these reagents used separately or in dual combinations with RBC but never all three at the same time. Any help will be greatly appreciated. Thanks. Ray Hester Univ. of South Alabama rhester@jaguar1.usouthal.edu
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:06 EST