Dr. Wes Chang of our place provided me this potential answer. GS-IB4 (Grifonia simplicifolia) and RCAI and RCA120 (from Ricinus communis) are two lectins that are reported to stain microglia in a variety of species, although I do not know about cat specifically. For antibodies, I found a message on the Histonet server that indicates DAKO's CR3/43 antibody labels microglia in human brain tissue. Again, don't know about cats. We, and others, make fluorescent GS-IB4. (If the image reproduces the green fluorescence is the microglia of rat brain, the red fluorescence Nissl substance and the blue fluorescence DAPI in nuclei.) [Image] "Richard K. Meister" wrote: > Hello, everyone: > > I have a colleague who wants to study microglial cells in cat brain. Do > any of you know of a marker for microglial cells? (I've looked, but so far > have only found MHC II markers used to study activated microglia.) We > would prefer an antibody that is specific for cat or cross-reacts, but any > other that we could test for cross-reactivity would also be helpful. > > The application would be to stain either parrafin-embedded tissue or frozen > sections. I would also like to hear about any markers that would work for > flow cytometry. > > Thanks in advance, > > Rick Meister > > * * * * * * * * * * * * * * * * * * * * * * * * * > * * * * * * * * * > * Richard K. > Meister Email: meister.1@osu.edu * > * The Ohio State University Voice: (614) > 292-9716 * > * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * > * Cytometry Instrumentation > Lab * > * 1925 Coffey > Road * > * Columbus, > OH 43210 U.S.A. * > * * * * * * * * * * * * * * * * * * * * * * * * * > * * * * * * * * *
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