If you do flow cytometry with lectins, beware of the problems caused by agglutination. For the GS-IB4, you are much better off using the GS-IA3B1 lectin, and blocking the activity of the A subunit with appropriate sugar. This yields a functionally monovalent probe and avoid agglutination of cells. For details, see: McCoy JP, Shibuya N, Riedy MC, and Goldstein IJ: Griffonia simplicifolia I Isolectin as a Functionally Monovalent Probe for Use in Flow Cytometry. Cytometry 7:142-146, 1986. -Phil J. Philip McCoy, Jr., Ph.D. Director of the Flow Cytometry Core Facility NHLBI - NIH Bldg 10, Rm 4A07 10 Center Dr., MSC 1357 Bethesda, MD 20892 > -----Original Message----- > From: Richard Haugland [mailto:richard.haugland@probes.com] > Sent: Thursday, November 21, 2002 7:54 PM > To: Cytometry Mailing List > Subject: Re: Microglial cell marker > > > Dr. Wes Chang of our place provided me this potential answer. > > > GS-IB4 (Grifonia simplicifolia) and RCAI and RCA120 (from > Ricinus communis) are > two lectins that are reported to stain microglia in a variety > of species, > although I do not know about cat specifically. For > antibodies, I found a > message on the Histonet server that indicates DAKO's CR3/43 antibody > labels microglia in human brain tissue. Again, don't know about cats. > > We, and others, make fluorescent GS-IB4. (If the image > reproduces the green > fluorescence is the microglia of rat brain, the red fluorescence Nissl > substance and the blue fluorescence DAPI in nuclei.) > > > [Image] > > > > "Richard K. Meister" wrote: > > > Hello, everyone: > > > > I have a colleague who wants to study microglial cells in > cat brain. Do > > any of you know of a marker for microglial cells? (I've > looked, but so far > > have only found MHC II markers used to study activated > microglia.) We > > would prefer an antibody that is specific for cat or > cross-reacts, but any > > other that we could test for cross-reactivity would also be helpful. > > > > The application would be to stain either parrafin-embedded > tissue or frozen > > sections. I would also like to hear about any markers that > would work for > > flow cytometry. > > > > Thanks in advance, > > > > Rick Meister > > > > * * * * * * * * * * * * * * * * * * * * > * * * * * > > * * * * * * * * * > > * Richard K. > > Meister Email: meister.1@osu.edu * > > * The Ohio State University Voice: (614) > > 292-9716 * > > * Dept. of Veterinary Biosciences FAX: (614) > 292-6473 * > > * Cytometry Instrumentation > > Lab * > > * 1925 Coffey > > Road > * > > * Columbus, > > OH 43210 U.S.A. * > > * * * * * * * * * * * * * * * * * * * * > * * * * * > > * * * * * * * * * >
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