Does anyone have any experience/opinion as to whether Fluorescein-5-EX succinimidyl ester would be a preferred choice over Fluorescein isothiocyanate (FITC) for conjugating a mouse monoclonal IgG Ab (for SIV gag antigen detection). Our concern is that since this antibody will be used for intracellular staining that there may be issues with the size difference between the two which may negate any advantages that the Fluorescein-EX would have over FITC. Your input is very much appreciated. Michelle Connole New England Primate Research Center Harvard University 1 Pine Hill Drive Southborough,MA 01772
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