Hi Adrian: I have been using Spherotech's UltraRainbow fluorescent particles (URFPs), they are great, they are 3.0 um particles that fluoresce across all three lasers (488, 633 and UV), are very stabile, and most important, very affordable! www.spherotech.com 847-680-8922 phone You prabably can get a free sample to try. Regards, Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu > From: Adrian Smith <A.Smith@centenary.usyd.edu.au> > Date: Fri, 6 Sep 2002 15:58:06 +1000 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: For Vantage(Diva) users #1 - beads? > > > To all the Vantage users out there (and those doing lots of colours), > > We are (still) trying to set up our Vantage (DiVA) for >6 > colours and we are wondering what people are using for "tweaking" > alignments, voltages etc for far red and UV fluorochromes. Last time > we checked with BD in Australia they didn't have any beads to > recommend or sell us. We have tried with various stained cells but we > are looking for something more reproducible for initial setup and > optimisation. > > We are also interested in getting some idea of the settings people > use and the levels we should be expecting from various flurochromes > so we have a rough baseline from which to work. > > At this stage we are completely open to using any combination of > fluorochromes - we just want to use more than 4! > > The Vantage is set up with three lasers (UV, 488, 633) and 8 > detectors (2,4,2) or which 3 or 4 are supposed to be the far-red type. > > Any comments and suggestions would be most welcome! > > Thanks, > > Adrian > > -- > ______________________________________________ > Adrian Smith (Research Officer) T CELL BIOLOGY GROUP > Centenary Institute of Cancer Medicine & Cell Biology > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > Ph: (02) 9565-6198 Fax: (02)-9565-6103 >
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