while we are discussing apoptosis scatter of T cells. I have a question regarding scatter of apoptotic neutrophils. I have attached a file showing the scatter of neutrophils in fresh blood and whole blood incubated with TNFa for 24h to induce apoptosis. CD16 shedding was used as the criteria to monitor apoptotic neutrophils. What I don't understand is that I look at CD16 staining on cells in Gate R1. When I set up a gate on Cd16-lo cells the pink population comes up. If I look at CD16 on cells in the pink population (low side and forward scatter) in the 24h incubated cells, the CD16 staining is coinciding with apoptotic peak (CD16lo). Can somebody please tell what can I conclude out of this. Also does it mean that we have to take into account the cells with a low side and forward scatter. Usually I gate on the CD16-lo population and look at that population for further events (like caspase 3 etc) I have attached a MAC file as well as a PC file so that both users can open it. Indresh Kaur Ph.D. Phone: 281-483-8791
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:13 EST