Hello all Just wondering if anyone has seen an effect of PFA fixation on SSC and FSC of clonal cell lines. I noramlly use live cells in FACS uptake assays but have recently begun fixing with 1%PFA (15 min) so that I can do analysis at a later date. However, I am now seeing two populations appear on the FSC/SSC plot.. I'm assuming the one with lower SSC/ FSC values are dead cells ??? Am also interested in advice on concentrations of PI and incubation times to use when using PI to asess cell viability many thanks as always, Annette Annette Byrne PhD Scientist Pharmacyclics 995 E Arques Ave Sunnyvale CA 94085-4521 USA Tel: 001-408-3283640 Fax: 001-408-3283689 e mail : AByrne@pcyc.com www.pharmacyclics.com
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