I appreciate your effort. However, I am not sure whether the false-positive rate in the publication might be too high for practical use, since I see much more CLL than mantle cell lymphoma. For example, the false-positive rate was 13% (4/30) for CLL and 31% (4/13) for splenic marginal zone lymphoma, although the latter is usually not difficult to distinguish from mantle cell lymphoma. With the current classification schemes a definitive diagnosis of mantle cell lymphoma essentially requires documentation of cyclin D1 over-expression or t(11;14) chromosomal translocation. I have been suggesting to the clinicians that specimens which might be mantle cell lymphoma be sent for FISH analysis for t(11;14). Tim Singleton, MD Flow Cytometry William Beaumont Hospital >>> ricardo morilla <ricardo@icr.ac.uk> 05/13/02 07:50AM >>> Dear All, The question of the detection of Cyclin D1 by flow has been discussed at length in this group during the past year. There are still people asking for a method to detect it. I enclose the method that we currently use in the lab and it is working in our hands. I hope that it will be of help to those of you who are trying to detect Cyclin D1 by flow. Ricardo. Reference: "Cyclin D1 by flow cytometry as a useful tool in the diagnosis of b-cell maliganacies" Manal O. Elnenaei et al. Leukaemia Research 25 (2001) 115-123 ========================================================== Mr. Ricardo Morilla Academic Department of Haematology & Cytogenetics The Royal Marsden Hospital 203 Fulham Road, London SW3 6JJ, UK Tel +44 (0) 20 7808 2882; Fax +44 (0) 20 7351 6420 ========================================================== *** eSafe scanned this email for malicious content *** *** IMPORTANT: Do not open attachments from unrecognized senders ***
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