Re: Cyclin D1 Method

From: Timothy Singleton, M.D. (tsingleton@smtpgw.beaumont.edu)
Date: Tue May 14 2002 - 07:45:22 EST


I appreciate your effort.  However, I am not sure whether the
false-positive rate in the publication might be too high for practical
use, since I see much more CLL than mantle cell lymphoma.  For example,
the false-positive rate was 13% (4/30) for CLL and 31% (4/13) for
splenic marginal zone lymphoma, although the latter is usually not
difficult to distinguish from mantle cell lymphoma.

With the current classification schemes a definitive diagnosis of
mantle cell lymphoma essentially requires documentation of cyclin D1
over-expression or t(11;14) chromosomal translocation.  I have been
suggesting to the clinicians that specimens which might be mantle cell
lymphoma be sent for FISH analysis for t(11;14).

Tim Singleton, MD
Flow Cytometry
William Beaumont Hospital

>>> ricardo morilla <ricardo@icr.ac.uk> 05/13/02 07:50AM >>>
Dear All,
The question of the detection of Cyclin D1 by flow has been
discussed at length in this group during the past year.
There are still people asking for a method to detect it.
I enclose the method that we currently use in the lab and
it is working in our hands.
I hope that it will be of help to those of you who are
trying to detect Cyclin D1 by flow.
Ricardo.

Reference:
"Cyclin D1 by flow cytometry as a useful tool in the
diagnosis of b-cell maliganacies" Manal O. Elnenaei et al.
Leukaemia Research 25 (2001) 115-123

==========================================================
Mr. Ricardo Morilla
Academic Department of Haematology & Cytogenetics
The Royal Marsden Hospital
203 Fulham Road, London  SW3 6JJ, UK

Tel    +44 (0) 20  7808 2882;  Fax    +44 (0) 20 7351 6420
==========================================================



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