RE: B cell antibodies

From: Stetler-Stevenso, Maryalice (NCI) (stetler@mail.nih.gov)
Date: Tue Jun 04 2002 - 08:08:59 EST

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Rituxan should not have done that. We have seen CD20 negativity after
treatment but the other B cell antibodies worked well. This unmarked
population could be something other than lymphoid or the CD19 and CD22
didn't make it into the tube (happens in the best of labs on occasion).

Maryalice Stetler-Stevenson, M.D., Ph.D.
Chief, Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH

Sometimes you're the windshield, sometimes you're the bug.

> ----------
> From:		avispeters@netscape.net
> Sent:		Monday, June 3, 2002 4:24 PM
> To:	Cytometry Mailing List
> Subject:	Re: B cell antibodies
>
>
> Speaking of Rituxan monitoring, we had a Dr. last week that ordered a CD20
> for Rituxan
> therapy monitoring and he wanted to know how many B cells were present.
> Looking at the
> total lymph gate, there were about 70% T cells (CD3+), about 8% NK cells
> (CD56+16+/CD3-)
> which would leave about 22% that should have been B cells. I could not get
> those B
> cells to stain with CD19, CD20, or CD22. There was always a population
> totally negative
> (0% for all 3 B markers) which amounted to about 20%. I thought the
> Rituxan was only
> supposed to block CD20. Could someone shed some light on this problem for
> me.
> Avis Peters
> United Hospital
> Flow Cytometry Lab
> St. Paul, MN
>
>
>
> "Timothy Singleton, M.D." <tsingleton@smtpgw.beaumont.edu> wrote:
>
> >
> >Cytoplasmic CD79a.
> >
> >Tim Singleton, MD
> >Flow Cytometry
> >Beaumont Hospital
> >Royal Oak, MI
> >
> >>>> Paula Fukushima <pif@mail.nih.gov> 05/24/02 09:40AM >>>
> >
> >Hi List,
> >    Does anyone have an idea of B cell antibodies to use to follow
> >disease
> >in B cell lymphomas
> >with patients now being given anti-CD22(Epratuzumab) and
> >anti-CD20(Rituxamab) besides CD19?
> >
> >	   Thanks, Paula
> >
> >
> >Paula Imus
> >Flow Cytometry
> >LP, NCI, NIH
> >10 Center Drive MSC-1500
> >Bethesda, Md. 20892-1500
> >
> >
> >
> >
>
>
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Next message: Julia Szeberenyi: "About Symposium & Workshop on Faces and Phases of Dendritic cells"
Previous message: Marty Bigos: "Re: How to enhance PE signal? or fluorochromes in general"
Maybe in reply to: Paula Fukushima: "B cell antibodies"
Next in thread: Stetler-Stevenso, Maryalice (NCI): "RE: B cell antibodies"
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