Dear Colleagues I have 2 flow cytometry clients; one needs to stain CD19 and the other needs to stain IgD, both on unfixed PBMCs. None of the conjugates which they have tried so far give good positive/negative discrimination. Does anyone have a recommendation for; a "good" directly conjugated anti human CD19 (any 488 excitable fluorochrome) a "good" FITC conjugated anti human IgD All suggestions greatly appreciated. Yours faithfully D.R. Lloyd MMed Sc CBiol MIBiol The Institute for Cancer Studies University of Birmingham Edgbaston Birmingham B15 2TT England Please use my central mailname address D.R.Lloyd@bham.ac.uk for correspondence. All others may not be delivered.
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