Hello, In addition to the bacterial growth inhibition property of AZIDE, there is a tendency to do without it when possible. This is due to its hazardous nature of causing explosions (when it reacts with metal waste drainage pipes). Some pipe fitters experienced this in Europe when hammering at the Azide contaminated pipes. This was the main reason why the manufacturers of Diluents used in Blood counting have opted for labeling these as AZIDE FREE. Hood luck. Fareed Al-Gurg Dubai Hospital Dubai - United Arab Emirates. ----- Original Message ----- From: Dr. Ashraf Abdelhafez To: Cytometry Mailing List Sent: Wednesday, September 25, 2002 4:28 PM Subject: Azide? Hi All: Does anybody have a conclusive answer to why azide is added to staining buffer. I have found information that says that it functions to inhibit microbial growth. I have found that it can be used to stop reactions. I have found people recommending not to use in certain experiment because it can alter protein function. And I have found people saying that you do not need to put if you are going to analyze the cells within hours. But I was not able to find information on that exact reason for adding it to staining buffer. I would like to hear what you have. Only what you believe is a conclusive answer is accepted. Thanks, Ashraf ------------------------------------------------------------------------------ Do you Yahoo!? New DSL Internet Access from SBC & Yahoo!
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