Dear Greg,
Please look in the May 2002 issue of Biotechniques for an excellent,
and quite simple, minimally disturbing hematopoietic cell synchrony method
(by Thornton et al). Using this method, the duration of synchrony is two to
three or more cycles and, (although not represented in the current
BioTechniques article), we also demonstrate that flow cytometric patterns of
protein expression during these multiple cycles of synchronous growth are
identical quantitatively and temporally to those in undisturbed asynchronous
culture.
Please feel free to contact me for more information.
Thank you,
Leigh Eward
Cell Biology
Florida Institute of Technology
(321) 674-8789
>From: Gregory Crawford <crawford@nhgri.nih.gov>
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>Subject: [cell synchrony technique]
>Date: Wed, 17 Apr 2002 15:14:58 -0400
>
>
>
>Hello,
>
>I am interested in synchronizing K562 lymphoblast cells and was
>interested in the most physiologically normal and effective method
>for achieving this. Does anyone have any experience with this?
>
>Thanks,
>Greg
>--
>********************
>Greg Crawford, Ph.D.
>National Human Genome Research Institute
>National Institute of Health
>Genome Technology Branch
>50 South Drive, Room 5309
>Bethesda, MD 20892
>
>tel: 301-594-9203
>fax: 301-480-9667
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