Dear Flowers, I want to thank everyone who responded to my question regarding the TUNEL assay. Many of the coments confirmed my own observations, with a few additional things I will try. The cell lines I am working with appear to stop DNA fragmentation at the 300-50kb sections. Therefore, I have to aquire and analyze them on a linear scale, which exaggerates any differences. I have recently controlled the absolute number of cells per sample, but I will also try to reduce the stress on the cells (I tend to vortex cells to mix them). I will also look into the "death detection kit", although I am somewhat limited on assays to use because the cells are already fixed. Thanks again, Shari Brezinsky Biogen
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