Dr. Hrusak, Very kind of you to write. I do appreciate your sending me this info. I'm encouraged by all of the responses I've gotten, and will try to go forward with this. Mark Pavlick > ---------- > From: Ondrej Hrusak > Sent: Monday, August 12, 2002 3:48 AM > To: Mark Pavlick; Cytometry Mailing List > Subject: Re: AB's for human perforin, granzymes > > Hello, > PE labelled Anti-perforin mAb from Pharmingen (BD) cat 556437 works OK for > us in NK cells. We are currently working on the CD8+ T cells that seem to > be more problematic. > K. Kogawa et al. used it for both NK and CTL with an apparent success: > http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=retrieve&db=pubmed&list_ > uids=11756153&dopt=Abstract > > Good luck, > Ondrej Hrusak > > > . . . . . . . . . . . . . . . . . . . . . . . . . . > Ondrej Hrusak, M.D., Ph.D. > Institute of Immunology > Ondrej.Hrusak@lfmotol.cuni.cz > fax +4202 2443 5962 > http://www.lf2.cuni.cz/clip/ > phone +4202 2443 5969, +4202 2443 2084 > postal address: V uvalu 84 > 150 06 Praha 5 > Czech Republic > > At 21:21 -0400 8.8.2002, Mark Pavlick wrote: > > > 8/8 > List members, > > > I want to stain human CD8 lymphocytes for the expression of > perforin and granzymes A and B. I'd appreciate any references to currently > available antibodies for this purpose. Thanks in advance. > > > > Mark Pavlick > > mvp@nih.gov > > >
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