I'm using the ISHAGE single-platform technique through use of Beckman-Coulter's Stem Kit. I use a stop count of 100K for CD45+ events and microspheres or a stop time of 10 minutes. After 10 minutes, the microspheres either float or sediment, requiring that the acquisition be paused, the tube removed to be vortexed and the sample acquisition resumed. Ideally, 100 events are analyzed. William "Do one thing every day that scares you." Eleanor Roosevelt ________________________________________________________________________ C. William King, Jr. FACS Core Research Facility Children's Research Institute, Room 5220 Children's National Medical Center, 111 Michigan Ave., NW Washington, District of Columbia 20010 202.884.2308 tel 202.884.5808 fax wking@cnmc.org _________________________________________________________________________ This email is confidential and intended solely for the use of the person(s), 'the intended recipient,' to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of Children's Research Institute or Children's National Medical Center. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly, any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are not the intended recipient please contact me, the sender, and dispose of this e-mail as soon as possible. _________________________________________________________________________ >>> "cy" <ooncy@pacific.net.sg> 11/04/02 01:30AM >>> Dear all, We usually acquired 75,000 CD45+ events for CD34 enumerations. We do not process the test if cell count is less than 1000/ul but there are occasions where 75,000 collection is not possible. I am interested to know what is the usual practice in other clinical labs when this happen? What is the minimum acceptable number of events collected to report CD34 count? Thank you. Ching Ying
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