Hi All! Is anyone using Quick Width as a Pulse Processing parameter for high speed sorting? I would be curious to know if you find it has improved the purity of your sorts. I am particularly interested in its utility in situations where you have cells that tend to stick together and you may get a positive cell and a negative cell stuck together when transversing the laser beam and it gets sorted together as a positive but when reanalyzed they are now separate cells which appears as a contaminate. I think this tends to be more of a problem when you are sorting based on one fluorescence parameter such as GFP. I would appreciate any opinions and experiences from those that have used this feature. Thanks as always-Joanne Joanne Lannigan, MS Director, Flow Cytometry Core Facility Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu
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