Oh, you B-cell immunologists. You assume that all colors are used for immunophenotyping. That's like our current philosophy, that B cells are good for only one thing: making reagents with which we can distinguish important T cell subsets! mr >Hi Michal, > >You are just another in the line of researchers who has an instrument which >has used a misleading terminology in its description. It does have three >lasers, and two are fairly useful although there is no compensation >circuitry between the 488 and 633 lasers (problem for APC and PE-Cy5 >channels). The third laser as mentioned above is a "UV", but the problem is >that the "UV" laser is too far down in the "UV" spectrum to be beneficial >for the "UV" excitable fluorochromes. So, unfortunately, this laser >configuration is totally useless when trying to use the currently available >fluorochromes to do 6-color staining. > >Randy T. Fischer >NIH/NIAMS >Building 10, Room 6D57 >9000 Rockville Pike >Bethesda, MD 20892 >(301) 594-3537 >fischer1@mail.nih.gov
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