Hi Michal and Randy, I assume Michal that you have a BD LSR? I also assume, Randy, that you have some sort of issue with it. To describe the laser configuration as 'totally useless' is not only provocative but also implies that 6 colour work is impossible on the LSR. Yes, the 325nm excitation isnt optimal for a lot of the dyes available but there is sufficient excitation to allow the use of Hoechst, DAPI, Indo, Alexa350 ands AMCA. I have certainly managed to do 6 colour combinations eg Indo (blue and green emission), FITC, PE, PE/Cy5 and APC or TP3. The 325nm laser may not be ideal and will presumably be replaced by a newer solid state 350ish one soon but I - and probably more importantly my users - have found it to be useful especially in doing analytical experiments that were once occupying my sorters. Derek On Thu, 31 Oct 2002, Fischer, Randy (NIH/NIAMS) wrote: > You are just another in the line of researchers who has an instrument which > has used a misleading terminology in its description. It does have three > lasers, and two are fairly useful although there is no compensation > circuitry between the 488 and 633 lasers (problem for APC and PE-Cy5 > channels). The third laser as mentioned above is a "UV", but the problem is > that the "UV" laser is too far down in the "UV" spectrum to be beneficial > for the "UV" excitable fluorochromes. So, unfortunately, this laser > configuration is totally useless when trying to use the currently available > fluorochromes to do 6-color staining. > Randy T. Fischer ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 Cancer Research UK, e_mail:derek.davies@cancer.org.uk London Research Institute, mobile: 07790 604112 44 Lincolns Inn Fields, London, UK. Web Page: http://sci.cancerresearchuk.org/axp/facs/davies/index.html In tenebris lux *************************************************************************
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